DICOM PS3.3 2021b - Information Object Definitions

A.32.8.4 VL Whole Slide Microscopy Image Functional Group Macros (Current)
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#### A.32.8.4 VL Whole Slide Microscopy Image Functional Group Macros

Table A.32.8-2 specifies the use of the Functional Group Macros used in the Multi-frame Functional Groups Module for the VL Whole Slide Microscopy Image IOD.

Table A.32.8-2. VL Whole Slide Microscopy Image Functional Group Macros

Functional Group Macro | Section | Usage
--- | --- | ---
Pixel Measures | C.7.6.16.2.1 | M - Shall be used as a Shared Functional Group.
Frame Content | C.7.6.16.2.2 | U - Shall not be used as a Shared Functional Group.
Referenced Image | C.7.6.16.2.5 | U
Derivation Image | C.7.6.16.2.6 | C - Required if the image or frame has been derived from another SOP Instance.
Real World Value Mapping | C.7.6.16.2.11 | U - May be used only if Photometric Interpretation (0028,0004) is MONOCHROME2.
Plane Position (Slide) | C.8.12.6.1 | C - Required if Dimension Organization Type (0020,9311) is not TILED_FULL; may be present otherwise.
Optical Path Identification | C.8.12.6.2 | C - Required if Dimension Organization Type (0020,9311) is not TILED_FULL; may be present otherwise.
Specimen Reference | C.8.12.6.3 | U
Whole Slide Microscopy Image Frame Type | C.8.12.9 | M - Shall be used as a Shared Functional Group.

### Note

The Plane Position (Slide) and Optical Path Identification Macros are Type C, which allows the Per-Frame Functional Group Sequence (5200,9230) to be entirely omitted in those cases in which there are no other Per-Frame Functional Group Macros with content (i.e., the Frame Content Macro is empty or absent).

##### A.32.8.4.1 VL Whole Slide Microscopy Image Functional Group Macros Content Constraints

###### A.32.8.4.1.1 Referenced Image

For Purpose of Reference Code Sequence (0040,A170) in the Referenced Image Functional Group DCID 8120 “WSI Referenced Image Purposes of Reference” shall be used.

###### A.32.8.4.1.2 Plane Position (Slide)

Frames shall occupy plane positions in a regular tiling of the Total Image Matrix; the frames may extend beyond the edges of the Total Image Matrix. Therefore, the value of Column Position In Total Pixel Matrix (0048,021E) shall be an integer multiple of the value of Columns (0028,0011) plus a constant, and the value of Row Position In Total Pixel Matrix (0048,021F) shall be an integer multiple of the value of Rows (0028,0010) plus a (possibly different) constant.

### Note

Pixels beyond the edge of the Total Image Matrix may be encoded with a fixed padding value. For images with MONOCHROME2 Photometric Interpretation, see Pixel Padding Value (0028,0120) in Section C.7.5.1.1.2.

The encoding of tiles may be sparse; i.e., some tiles may not be encoded in frames. There are no constraints on the ordering of frames within the pixel data; each frame specifies its position in the Plane Position (Slide) Functional Group.

### Note

Different SOP Instances within a Series may have different tiling boundaries and sizes.

Frames associated with different optical paths may coincide in any spatial dimension.

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DICOM PS3.3 2021b - Information Object Definitions